Construction of a vector for coupled in vitro transcription/translation.

champ et al. (2), in which the total isolated DNA from each sample serves as a standard, our method uses an internal competitor and is consequently not affected by host muscle size variability. Furthermore, the variability in DNA extraction efficiency should be rendered insignificant because the competitor plasmid is affected in the same extent as the genomic DNA. This new method can be applied to follow transplantation of any cell type and can be adapted for the precise quantification over a large spectrum of experiments.